Abstract In this study, antibacterial, antioxidant and phenolic compounds of raisin extracts was evaluated. Different solvent extraction methods were utilized to extract phenolic compounds from raisin. The phenolic compounds of raisin extracts was determined using HPLC and three compounds were found, catechin, quercetin and rutin. The antibacterial activity of the acetonic extract was tested against four bacterial pathogens viz. Staphylococcus aureus, Pseudomonas aeroginosa, Salmonella typhimurium and Escherichia coli using both disk diffusion and minimum inhibitory concentration (MIC) methods. The acetonic extract exhibited the highest activity against Staphylococcus aureus with zone of inhibition of 14mm and the MIC of 25 mg/ml. However, there was a little activity against E. coli and S. typhimurium. The methanolic extract showed good antioxidant activity as indicated by 2,2-diphenyl-1-picrylhydrazyl free radical assay (DPPH). In conclusion, raisin contains potent phenolic compounds and their application might function as promising natural preservative and antimicrobial agents in food industry and also good antioxidant food that helps to minimize the risk of degenerative diseases. arabic 11 English 86
Abdulwahab Kammon, Yousef M. Abouzeed, Faraj Zgheel, Abdurrezagh Abdalla Elfahem, Mohammed Saad Almagarhe, Abdulgader Dhawi, Abdulkareem Elbaz, Murad A. Hiblu, Mohamed O. Ahmed (12-2018)

Aim: The aim of this work was to isolate and molecularly identify enterohemorrhagic Escherichia coli (EHEC) O157 in milk and dairy products in Libya, in addition; to clear the accuracy of cultural and biochemical identification as compared with molecular identification by partial sequencing of 16S rDNA for the existing isolates. Materials and Methods: A total of 108 samples of raw milk (cow, she-camel, and goat) and locally made dairy products (fermented cow’s milk, Maasora, Ricotta and ice cream) were collected from some regions (Janzour, Tripoli, Kremiya, Tajoura and Tobruk) in Libya. Samples were subjected to microbiological analysis for isolation of E. coli that was detected by conventional cultural and molecular method using polymerase chain reaction and partial sequencing of 16S rDNA. Results: Out of 108 samples, only 27 isolates were found to be EHEC O157 based on their cultural characteristics (Tellurite-Cefixime-Sorbitol MacConkey) that include 3 isolates from cow’s milk (11%), 3 isolates from she-camel’s milk (11%), two isolates from goat’s milk (7.4%) and 7 isolates from fermented raw milk samples (26%), isolates from fresh locally made soft cheeses (Maasora and Ricotta) were 9 (33%) and 3 (11%), respectively, while none of the ice cream samples revealed any growth. However, out of these 27 isolates, only 11 were confirmed to be E. coli by partial sequencing of 16S rDNA and E. coli O157 Latex agglutination test. Phylogenetic analysis revealed that majority of local E. coli isolates were related to E. coli O157:H7 FRIK944 strain. Conclusion: These results can be used for further studies on EHEC O157 as an emerging foodborne pathogen and its role in human infection in Libya.
Ibrahim Eldaghayes(11-2016)

Poor performance in athletic horses due to musculoskeletal affections is very common in Libya. Two mature thoroughbred horses suffering from poor performance and history of moderate degree forelimb lameness were admitted to the Aswany Private Equine clinic in Tripoli, Libya. The study is aiming for presenting a simple, time effective, cost effective surgical procedure to treat distal simple splint bone fracture in thoroughbred horses. Horses were examined thoroughly for the common muscle, hoof, and tendons affections. A progressed unilateral left forelimb swelling involving the lateral distal part of the fourth metacarpal bones was noticed. Three radiographs were taken to each horse including latero- medial, dorsolateral-palmaromedial, and palmarolateral-dorsomedial views to assess both splint bones. The radiographs showed that there is a complete fracture involving the distal extremity (button) of the fourth metacarpal bones. Surgical excisions of the fractured part of the splint bones have been decided to treat these horses. To precisely locate the site of the bone fragments, 1.5 mm stainless steel wires were placed over the distal part of splint prior to taking the radiographs. The surgical sites were aseptically prepared, and the horses were then sedated via intravenous injection of xylazine at a dose rate of 1 mg/kg body weight. The site of surgical incision was infiltrated with 2% lidocaine. The bone fragments were then removed after performing the surgical incision. The sharp end at the distal end of the proximal part of the splint bone was then smoothening with a scissor. The surgical incisions were then routinely closed and the post-operative care and radiographs were taken. The surgical incision made complete healing within two weeks. Both horses were followed up for up to three years post-operation, and they did well compared to what has been done in other studies. We concluded that the surgical technique used to treat the two horses with splint bone fracture enrolled in the current study is a simple and effective procedure. arabic 19 English 106
Mohamed H. Abushhiwa, Osma K Sawesi, Khaled A. Milad, Aiman. A. Shalgum, Abdulrhman Mohamed Salah Alrtib(9-2019)