Background and Aim: Foodborne illnesses are a serious challenge to human health and the economic sector. For example, salmonellosis remains a burden in developed and developing nations. Rapid and reliable molecular methods to identify Salmonella strains are essential for minimizing human infection. This study aimed to identify Salmonella spp. in raw milk and dairy products using conventional and molecular techniques and to test the antibiotic susceptibility of the isolated strains. Materials and Methods: One hundred and thirty-one milk and dairy product samples were randomly collected from different localities in Libya. Samples were examined for the presence of Salmonella by conventional culture techniques, including cultivation in Rappaport-Vassiliadis broth and streaking on xylose lysine deoxycholate agar. Identification also used polymerase chain reaction and partial sequencing of 16S rDNA. Twenty-four antibiotics were used for the examination of antimicrobial resistance of Salmonella spp. isolates with the agar disk diffusion method (Kirby–Bauer technique). Multi-antibiotic resistance index and antibiotic resistance index (ARI)for Salmonella enterica isolates were calculated. Results: Twenty-one of 131 samples (16%) were positive for Salmonella spp. recovered from 9 (16%), 2 (11%), 4 (22.2%), and 6 (46%) samples of raw cow milk, fermented raw milk, and fresh locally made soft cheeses, Maasora and Ricotta), respectively. Samples of ice cream, milk powder, and infant formula showed no Salmonella spp. contamination. Only 9 of 21 (42.8%) isolates were confirmed as S. enterica by partial sequence 16S rDNA analysis. All isolates were resistant to amoxycillin, bacitracin, penicillin G, lincomycin, vancomycin, clindamycin,
Salah M. Azwai(5-2022)

Vancomycin-resistant enterococci (VRE) are both of medical and public health importance associated with serious multidrug-resistant infections and persistent colonization. Enterococci are opportunistic environmental inhabitants with a remarkable adaptive capacity to evolve and transmit antimicrobial-resistant determinants. The VRE gene operons show distinct genetic variability and apparently continued evolution leading to a variety of antimicrobial resistance phenotypes and various environmental and livestock reservoirs for the most common van genes. Such complex diversity renders a number of important therapeutic options including "last resort antibiotics" ineffective and poses a particular challenge for clinical management. Enterococci resistance to glycopeptides and multidrug resistance warrants attention and continuous monitoring.
Mohamed O. Ahmed , Keith E Baptiste (6-2017)

Aims: The aim of the study was to characterize a spirochaete isolated from thelesions of a cow with digital dermatitis (DD).Methods and Results: The characterization was on the basis of its light andelectron microscopic appearance, enzymic profile and DNA sequence analysisof its flagellin and 16S rRNA genes. The spirochaete was 6–8-lm long and 0Æ2–0Æ3 lm in diameter, and possessed seven to eight periplasmic flagella, withthree to five helical turns. The enzymic profile of the bacterium resembles, butis not identical to that of Treponema brennaborense. Its flagellin gene sequencewas identical to that of Treponema phagedenis but distinct from that of anovine spirochaete. Analysis of a 1477-bp region of the 16S rRNA genes indica-ted that this is a Treponema species and that it is indistinguishable from someisolates made from cases of bovine DD in the United States. Finally, electronmicroscopy revealed the presence of myovirus-like bacteriophage particles in allcultures of the treponeme examined.Conclusions: The spirochaete isolate was identified as a Treponema species clo-sely related to some isolates from the United States (by 16S rDNA) and to T.phagedenis (by flagellin gene sequence) and is associated with bacteriophageparticles.Significance and Impact of the Study: The fact that the isolates with the sameor very similar 16S rDNA sequences have been obtained from cases of bovineDD in cattle in different countries at different times, lends further support tothe hypothesis that treponemes play a role in the pathogenesis of this disease arabic 10 English 72
Abdulgader Dhawi Alfitouri Dhawi(1-2021)