This study was conducted to investigate the presence of Enterococcus spp. in meat, meat products and seafood. A hundred and four samples were randomly collected from different geographic localities in Libya. The samples were subjected to microbiological analysis for enumeration and isolation of Enterococcus spp. by conventional cultural and molecular identification using PCR and partial sequencing of 16S rDNA techniques. Out of 104 samples, 73 (70.2%) isolates were found to be enterococci based on their cultural characteristics on ESD medium. However, out of 36 samples subjected to molecular identification, only six isolates were confirmed to be Enterococcus spp. using PCR and partial sequencing of 16S rDNA technique. All enterococci strains tested for their antibiotic sensitivity profiles showed high percentage of multi-resistance phenotype. These results can be used for further studies on enterococci as an emerging food borne pathogen and its role in human infection in Libya and would suggest that meat, meat products and seafood might play a role in the spreading of enterococci through the food chain with antimicrobial resistance characteristics.
Ibrahim Eldaghayes(6-2017)

This study aimed to evaluate the antibacterial activity of flavonoids extracted from two Libyan brown algae namely Cystoseira compressa and Padina pavonica using microwave-assisted extraction method against pathogenic bacteria isolated from meat, meat products, milk and dairy products (Staphylococcus aureus subsp. aureus (5 isolates), Bacillus cereus (3 isolates), Bacillus pumilus (1 isolate), Salmonella enterica subsp. enteric (4 isolates) and Enterohaemor-rhagic Escherichia coli O157 (EHEC O157) (4 isolates)). All of these isolates were muti-drug resistant with high MAR index. The results showed that C. compressa extract exhibited better and stronger antibacterial activities against the seventeen tested isolates with inhibition zones diameter ranged from 14 - 22 mm compared to P. pavonica extract which showed positive effect against 9 isolates with low inhibition zone ranged from 11 - 16.5 mm. Flavonoids extracted from C. compressa also displayed the best spectrum of bactericidal effect with a ratio MBC/MIC ≤ 4 obtained on all susceptible tested bacterial strains. Flavonoids and proanthocyanidins significantly contributed to the antibacterial properties. The mode of action of these active extracts is under investigation.
Ibrahim Eldaghayes(1-2017)

Paper currency is one of the potential vectors to transmit diseases, since banknotes continuously pass through different hands, as a medium of exchange, which increases the possibility of transferring pathogenic microorganisms and thus cross contamination. This study was carried out on one hundred and ninety-eight paper currency notes that were collected randomly in the city of Tripoli, Libya, from different communities namely, petrol and gas stations, supermarkets, mini markets, cafeterias and fast food restaurants, etc. The samples consist of four pieces, each of currency (Dirham) denominations (250, 500, 1.000 and 5.000 notes). Conventional and current microbiological techniques were used in order to isolate and identify the species of microorganisms that resulted in the following percentages: Enterobacter agglomerans 19%, Pseudomonas spp. 16%, S. aureus, Enterobacter cloacae 11%, Klebsiella pneumonia 11% and E. coli 10%. These results indicate that Libyan banknotes are commonly contaminated with pathogenic bacteria, which have the possibility of spreading human diseases. Higher denomination currency showed lower contamination. Consequently, great care should be taken during the handling of money for the safety of human health. arabic 10 English 65
Abdulgader Dhawi Alfitouri Dhawi(1-2021)