Poor performance in athletic horses due to musculoskeletal affections is very common in Libya. Two mature thoroughbred horses suffering from poor performance and history of moderate degree forelimb lameness were admitted to the Aswany Private Equine clinic in Tripoli, Libya. The study is aiming for presenting a simple, time effective, cost effective surgical procedure to treat distal simple splint bone fracture in thoroughbred horses. Horses were examined thoroughly for the common muscle, hoof, and tendons affections. A progressed unilateral left forelimb swelling involving the lateral distal part of the fourth metacarpal bones was noticed. Three radiographs were taken to each horse including latero- medial, dorsolateral-palmaromedial, and palmarolateral-dorsomedial views to assess both splint bones. The radiographs showed that there is a complete fracture involving the distal extremity (button) of the fourth metacarpal bones. Surgical excisions of the fractured part of the splint bones have been decided to treat these horses. To precisely locate the site of the bone fragments, 1.5 mm stainless steel wires were placed over the distal part of splint prior to taking the radiographs. The surgical sites were aseptically prepared, and the horses were then sedated via intravenous injection of xylazine at a dose rate of 1 mg/kg body weight. The site of surgical incision was infiltrated with 2% lidocaine. The bone fragments were then removed after performing the surgical incision. The sharp end at the distal end of the proximal part of the splint bone was then smoothening with a scissor. The surgical incisions were then routinely closed and the post-operative care and radiographs were taken. The surgical incision made complete healing within two weeks. Both horses were followed up for up to three years post-operation, and they did well compared to what has been done in other studies. We concluded that the surgical technique used to treat the two horses with splint bone fracture enrolled in the current study is a simple and effective procedure. arabic 19 English 106
Mohamed H. Abushhiwa, Osma K Sawesi, Khaled A. Milad, Aiman. A. Shalgum, Abdulrhman Mohamed Salah Alrtib(9-2019)

The genus Vibrio includes several food-borne pathogens that cause a spectrum of clinical conditions including septicemia, cholera and milder forms of gastroenteritis. Several Vibrio spp. are commonly associated with food-borne transmission including Vibrio cholerae, Vibrio parahemolyticus, and Vibrio vulnificus. Microbiological analysis for enumeration and isolation of Vibrio spp. were carried out for a total of 93 samples of seafood, meat and meat products from different geographic localities in Libya (Tripoli, Regdalin, Janzour and Toubruk). Vibrio spp. were detected by conventional cultural and molecular method using PCR and sequencing of 16S rDNA. Out of the 93 cultured samples only 48 (51.6%) yielded colonies on Thiosulfate Citrate Bile Salt agar (TCBS) with culture characteristics of Vibrio spp. More than half (n=27) of processed seafood samples (n=46) yielded colonies on TCBS, while only 44.6 % of samples of meat and meat products showed colonies on TCBS. Among cultured seafood samples, the highest bacterial count was recorded in clam with a count of 3.8 х104 CFU\g. Chicken burger samples showed the highest bacterial count with 6.5 х104 CFU\g. Molecular analysis of the isolates obtained in this study, showed that 11 samples out of 48 (22.9%) were Vibrio spp. Vibrio parahemolyticus was isolated from camel meat for the first time. This study is an initial step to provide a baseline for future molecular research targeting Vibrio spp. foodborne illnesses. This data will be used to provide information on the magnitude of such pathogens in Libyan seafood, meat and meat products.
Salah M. Azwai(1-2016)

The 11p15 region is organised into two independent imprinted domains controlled by imprinting control regions, which carry opposite germline imprints. Dysregulation of 11p15 genomic imprinting results in two human fetal growth disorders (Silver-Russell syndrome (SRS, MIM 180860) and Beckwith-Wiedemann syndrome (BWS, MIM 130650)) with opposite growth phenotypes. The mouse orthologous region on distal chromosome 7 (dist7) is well conserved in its organisation and its regulation. Targeted mutagenesis in mice has provided highly valuable clues in terms of the mechanisms involved in the regulation of genomic imprinting of the 11p15/dist7 imprinted region. On the other hand, the recent identification of unexpected genetic defects in BWS and SRS patients also brought new insights into the mechanisms of 11p15 imprinting regulation. However, some mouse models and human genetic defects show contradictions in term of growth phenotypes and parental transmission. In this review, we extensively analyse those various mouse and human models and more particularly models with mutations affecting the two imprinting centres, in order to improve our understanding of regulation of 11p15/dist7 genomic imprinting. arabic 21 English 117
Mansur Ennuri Moftah Shmela, C. F. Gicquel(1-2013)