Foot-and-mouth disease viruses are often restricted to specific geographical regions and spread to new areas may lead to significant epidemics. Phylogenetic analysis of sequences of the VP1 genome region of recent outbreak viruses from Libya and Saudi Arabia has revealed a lineage, O-Ind-2001, normally found in the Indian subcontinent. This paper describes the characterization of field viruses collected from these cases and provides information about a new real-time RT-PCR assay that can be used to detect viruses from this lineage and discriminate them from other endemic FMD viruses that are co-circulating in North Africa and western Eurasia.
Ibrahim Eldaghayes(12-2014)

Poor performance in athletic horses due to musculoskeletal affections is very common in Libya. Two mature thoroughbred horses suffering from poor performance and history of moderate degree forelimb lameness were admitted to the Aswany Private Equine clinic in Tripoli, Libya. The study is aiming for presenting a simple, time effective, cost effective surgical procedure to treat distal simple splint bone fracture in thoroughbred horses. Horses were examined thoroughly for the common muscle, hoof, and tendons affections. A progressed unilateral left forelimb swelling involving the lateral distal part of the fourth metacarpal bones was noticed. Three radiographs were taken to each horse including latero- medial, dorsolateral-palmaromedial, and palmarolateral-dorsomedial views to assess both splint bones. The radiographs showed that there is a complete fracture involving the distal extremity (button) of the fourth metacarpal bones. Surgical excisions of the fractured part of the splint bones have been decided to treat these horses. To precisely locate the site of the bone fragments, 1.5 mm stainless steel wires were placed over the distal part of splint prior to taking the radiographs. The surgical sites were aseptically prepared, and the horses were then sedated via intravenous injection of xylazine at a dose rate of 1 mg/kg body weight. The site of surgical incision was infiltrated with 2% lidocaine. The bone fragments were then removed after performing the surgical incision. The sharp end at the distal end of the proximal part of the splint bone was then smoothening with a scissor. The surgical incisions were then routinely closed and the post-operative care and radiographs were taken. The surgical incision made complete healing within two weeks. Both horses were followed up for up to three years post-operation, and they did well compared to what has been done in other studies. We concluded that the surgical technique used to treat the two horses with splint bone fracture enrolled in the current study is a simple and effective procedure. arabic 19 English 106
Mohamed H. Abushhiwa, Osma K Sawesi, Khaled A. Milad, Aiman. A. Shalgum, Abdulrhman Mohamed Salah Alrtib(9-2019)

This study aimed to evaluate the antibacterial activity of flavonoids extracted from two Libyan brown algae namely Cystoseira compressa and Padina pavonica using microwave-assisted extraction method against pathogenic bacteria isolated from meat, meat products, milk and dairy products (Staphylococcus aureus subsp. aureus (5 isolates), Bacillus cereus (3 isolates), Bacillus pumilus (1 isolate), Salmonella enterica subsp. enteric (4 isolates) and Enterohaemor-rhagic Escherichia coli O157 (EHEC O157) (4 isolates)). All of these isolates were muti-drug resistant with high MAR index. The results showed that C. compressa extract exhibited better and stronger antibacterial activities against the seventeen tested isolates with inhibition zones diameter ranged from 14 - 22 mm compared to P. pavonica extract which showed positive effect against 9 isolates with low inhibition zone ranged from 11 - 16.5 mm. Flavonoids extracted from C. compressa also displayed the best spectrum of bactericidal effect with a ratio MBC/MIC ≤ 4 obtained on all susceptible tested bacterial strains. Flavonoids and proanthocyanidins significantly contributed to the antibacterial properties. The mode of action of these active extracts is under investigation.
Ibrahim Eldaghayes(1-2017)