The effect of low and high doses of Salmonella enteritidis PT4 (SE) on immunocompetent cells in caecum and spleen of one-day-old chicks was investigated. Subsets of T lymphocytes positive for CD3, CD4, CD8 and B lymphocytes (Bu1b-positive cells) were counted in the caecum after immunohistochemical staining and the relative percentage of these cells in the spleen was analysed using a FACScan cytometer on days 7, 10, 14, 21, and 27 post-inoculation (pi). In the low dose group, the number of CD3+ and CD4+ cells in the caecum had significantly increased at day 10 pi. Both CD8+ and Bu1b+ cells were significantly higher on day 14 pi in this group. In the high-dose group, the number of CD4+ cells had significantly increased at day 7 pi. CD3+, CD8+, and Bu1b+ cells showed prolonged proliferation at days 7 up to 21 pi. Splenic lymphocytes demonstrated significant changes only in the high dose group. The percentage of splenic CD4+ cells was decreased at day 7 pi. A decrease in CD3+ and CD8+ cells was found at day 14 pi in this group.
Abdulatif Asheg(11-2002)

The present study aimed at evaluation of the immuomodulatory effect of sulphadimidine by investigating its ability to affect constitutive expression of MHC-II molecules on B-cells, which are one determinant of antigen presentation and the vigor of immunity. Three groups of broiler chicken were used, the first two groups received 100 mg/kg and 200 mg/kg sulfadimidine, respectively, in drinking water; while the third group was left as control. Bursal samples were taken after 7 and 14 days of treatment and checked for MHC-II gene expression by RT-PCR technique. The obtained results demonstrate that oral administration of sulphadimidine inhibited the expression of MHC-II mRNA in bursal of Fabricius of broiler chicken in a dose and age dependent manner. This finding is of clinical importance as many farmers routinely add sulpha drugs to drinking water as a prophylactic measure against some infections; yet the data presented in this study doesn't recommend this managing behavior. arabic 11 English 83
Marwan Draid(5-2012)

Detection and molecular characterization of infectious laryngeotracheitis in laying hen in Libya
Abdulatif Asheg(9-2013)