The imprinted expression of the IGF2 and H19 genes is controlled by the imprinting control region 1 (ICR1) located at chromosome 11p15.5. DNA methylation defects involving ICR1 result in two growth disorders with opposite phenotypes: an overgrowth disorder, the Beckwith-Wiedemann syndrome (maternal ICR1 hypermethylation in 10% of BWS cases) and a growth retardation disorder, the Silver-Russell syndrome (paternal ICR1 loss of methylation in 60% of SRS cases). In familial BWS, hypermethylation of ICR1 has been found in association with microdeletion of repetitive DNA motifs within ICR1 that bind the zinc finger protein CTCF; but more recently, ICR1 point mutations were described in BWS pedigrees. We present a case report of two brothers with BWS and prolonged post-pubertal growth resulting in very large stature. A maternally inherited point mutation was identified in ICR1 in both brothers, which altered binding of OCT transcription factors. The same mutation was present on the paternally inherited allele of their unaffected mother. This is a second report of a point mutation causing ICR1 hypermethylation by altering an OCT-binding motif. The atypical growth phenotype of the brothers may be connected to the unusual underlying cause of their BWS. arabic 24 English 118
Rebecca L Poole, Donald J Leith, Louise E Docherty, Mansur Ennuri Moftah Shmela, Christine Gicquel,, Miranda Splitt, I Karen Temple, Deborah J G Mackay(2-2012)

Peste des Petits Ruminants (PPR) is considered one of the most important transboundary animal diseases (TADs) with socio-economic impacts on national and international levels. During 2015-2016 a total of 690 serum samples were collected from unvaccinated domestic ruminants of which 555 sheep, and 135 goats representing teen provinces distributed in four Libyan branches (Green Mountain, Benghazi, West Mountain and Sabha). The sample were analysed at IZSAM, Teramo, Italy, by using competitive ELISA, IDvet innovative diagnostics (IDvet, 310 rue Louis Pasteur-34790 Grabels, France). The overall sero-prevalence rate (SPR) of PPR antibodies was estimated to be 41% (95% CL: 36% to 46%) among sheep and 39% (95% CL: 28% to 45%) among goat. A chi-square test was used to evaluate the probability of differences observed among SPR of infection. The results showed that the SPR of PPR was significantly (P= 0.015) higher in adult animals 41% (95% CL= 37%-46%) than in the young 24% (95% CL= 17%- 32%). The highest SPR 75% (95% CL= 61%-85%) was recorded in Sabha province (Southern Libya) which highlighted statistically difference (P= 0.00001). The preliminary results of the present study could be useful to better focus on specific area of Libya to improve understand and evaluate the risk factors for disease spreading and to plan disease control activities as requested by FAO/OIE (PPR Global Control and Eradication Strategy). arabic 19 English 100
Abdusalam Sharef Abdusalam Mahmoud(1-2020)

Investigation on the presence of antibodies against Chicken Infectious Anemia in Libya using the Enzyme-Linked Immunosorbent Assay
Abdulatif Asheg(4-2005)