Efficient extraction of genomic DNA (gDNA) from biological materials found in harsh environments is the first step for successful forensic DNA profiling. This study aimed to evaluate two methods for DNA recovery from animal tissues (livers, muscles), focusing on the best storage temperature for DNA yield in term of quality, quantity, and integrity for use in several downstream molecular techniques. Six male Swiss albino mice were sacrificed, liver and muscle tissues (n=32) were then harvested and stored for one week in different temperatures, -20C, 4C, 25C and 40C. The conditioned animal tissues were used for DNA extraction by Chelex-100 method or NucleoSpin Blood and Tissue kit. The extracted gDNA was visualized on 1.5% agarose gel electrophoresis to determine the quality of gDNA and analysed spectrophotometrically to determine the DNA concentration and the purity. Both methods, Chelex-100 and NucleoSpin Blood and Tissue kit found to be appropriate for yielding high quantity of gDNA, with the Chelex100 method yielding a greater quantity (P < 0.045) than the kit. At -20C, 4C, and 25C temperatures, the concentration of DNA yield was numerically lower than at 40C. The NucleoSpin Blood and Tissue kit produced a higher (P=0.031) purity product than the Chelex-100 method, particularly for muscle tissues. The Chelex-100 method is cheap, fast, effective, and is a crucial tool for yielding DNA from animal tissues (livers, muscles) exposed to harsh environment with little limitations.
Huda H. Al-Griw, Zena A. Zraba, Salsabiel K. Al-Muntaser, Marwan M. Draid, Aisha M. Zaidi, Refaat M. Tabagh , Mohamed A. Al-Griw(8-2017)

يستخدم الجادولنيوم كعامل لتحسين تباين صور الرنين المغناطيسي في أغلب حالات التصوير التي يتعذر فيها تحديد نوع الاصابة او طبيعة الورم، ويتميز الجادولنيوم بخصائص مغناطيسية تمكنهُ من التأثير على زمن الاسترخاء للبروتونات الموجودة في النسيج. مما يسبب في زيادة شدة الاشارة أو تقليلها حسب نوع الصورة. ويهدف هذا البحث لدراسة العلاقة بين زيادة شدة الإشارة في أنسجة الدماغ بعد استخدام الجادولنيوم وبعض البارامترات المحسوبة إحصائيا عن طريق معالجة صور الرنين باستخدام طريقة مصفوفة التكرارات للمستويات الرماديةGrey Level Co-occurrence Matrix. وحيث أن زيادة شدة الاشارة تكون نتيجة تقصير زمن الاسترخاء في الصورة المرجحة T1. تم دراسة إحدى أهم العوامل المؤثرة في الإشارات المكونة لصورة الرنين المغناطيسي وهو زمن الاسترخاء، وتم توضيح تأثير زمن الاسترخاء الطولي T1 والعرضي T2على تباين انسجة الدماغ في صور الرنين، ثم قمنا بمعالجة صور مرجحة لزمن الاسترخاء الطولي (T1- Weighted) أُخدت في المقطع المحوري (axial)، هذه الصور لحالات مصابة بأورام في الدماغ تم فيها استعمال الجادولنيوم. اهتمت هذه الدراسة بتحديد العلاقة بين زيادة شدة الاشارة في الانسجة المصابة وبعض البارامترات المحسوبة احصائياً عن طريق المعالجة. كما تم دراسة مدى استجابة الانسجة الطبيعية للجادولنيوم وتم مقارنتها بالأنسجة المصابة. واثبتت النتائج ان زيادة شدة الإشارة بعد استعمال حُقن الجادولنيوم في المناطق التي تحتوي على أنسجة مصابة يزيد في قيم البكسل المكونة لصورة الرنين. وبينت النتائج أن زيادة شدة الإشارة تتناسب مع تلك البارامترات المحسوبة كُلا حسب طبيعة النسيج. Abstract Gadolinium used as a factor to improve the contrast magnetic resonance images, in most cases of imaging that can't determine the type of lesion or tumor. Gadolinium is characterized by magnetic properties of being able to influence the relaxation time of protons in tissue. This would cause an increase or decrease in the intensity of the signal depending on the type of image. This research aims to study the relationship between the increases in signal intensity in the brain tissue by using Gadolinium. Statistically method would be implied after resonance images are processed, using a matrix method of iterations for gray level, it is know as Grey Level Co-occurrence Matrix. In T1-weighted image, an increase in signal intensity is duo to shorting of relaxation time. Relaxation time which is considered as one of the most important aspect effecting the signal component from MRI images was studied in depth. Also, the effect of relaxation time longitudinal T1 and transverse T2 on the variation of brain tissue were clarified. Then, T1-weighted axial images were processed. Images used in this work were taken from patients with brain tumor, and contrast agent (Gadolinium) was used to enhance them. This study focused on identifying the relationship between the increase in signal intensity in the lesion tissues and statistically calculated parameters. The respond of normal and effected tissues the Gadolinium were compared. The study shows that an in increases in pixel intensity, after contrast is introduced, in areas with infected tissue. Also, the results indicate that signal intensity commensurate with those calculated parameters.
تهاني عياد صالح العباني (2014)

Abstract Biological studies of Nezara viridula (L.) (Heteroptera:Pentatomidae) and its parasitoid Trissolcus basalis (Wollaston)(Scelionidae: Hymenoptera) collected from Libya were conducted in a rearing room at 25 ± 5°C, 65 ± 10 % R.H. and 16:8 LD photoperiod.During the course of rearing of N. viridula the eggs incubation periodvaried between 4-7 days. The newly emerged nymphs and the 5th stage had the highest mortality in comparison with 1st, 2nd, 3rd and 4thstages .studies on T. basalis included: SEM, development time, parasitoid mating behavior and oviposition behavior, functionalresponse and fecundity. The development time was 4 to 10 days underthe laboratory condition. Adult males lived longer than females.Mating time took from 12.2 to 19.5 seconds. In case of ovipositionbehavior serial of behaviors were observed and recorded. The egg selection period was 0: 00: 08 - 0: 00: 25 min, oviposition period was 0: 01: 04 - 0: 01: 41 min. Egg marking period was 0: 00: 11 - 0: 00: 25 min. The longest period was the oviposition period followed by egg marking period followed by egg selection period.In functional response study was found a highly positive correlation ( r = 0.974 ) number of egg masses exposed and the number of parasitoid emerged. Fecundity experiments showed that the daily ii production of progeny by female of T. basalis during 6-10 dayslifetime showed that female progeny production was higher than male progeny production. The maximum period offspring production was 6-10 days. There was a highly negative correlation ( r = - 0.900 ) between the number of eggs parasitized and parasitoid age . Also there is a highly signifcant difference between the fecundity of T. basalis newly emerged of 24 h and after 7 days after emergence from N. viridula eggs. The number of mature eggs in the ovary were less in 24h aged female and the fecundity increased over the next 7 days.
عايدة عادل عبد الرحيم بادي (2008)