Several molecular techniques that are commercially described in the literature for early detection of blood stream infection in attempting to overcome the limitations of the gold standard blood culture. SepsiTest™ is aCE marked commercial platform that has been described in the literature. However, there were no studies addressing the accuracy of the test as a diagnostic platform for detection bacteraemia in whole blood samples. The study, conducted to investigate and discuss the strategy of SepsiTest™ in comparisonto our previously validated real-time PCR based assays (BactScreen™) and the previously published “in-house” minor groove binder (MGB)-based all bacteria assay. The three assays showed different sensitivity patterns for detecting bacteria in blood stream pathogens in favouring of using BactScreenTMtest. SepsiTest™ could be valuable but their lowest sensitivity in addition to their use of the unspecific SYBR Green fluoresce dye that question its diagnostic accuracy could be used as a last choice molecular diagnostic technique for detection of bacteraemia in whole blood samples. However, SepsiTest™ strategy rather, may provide useful diagnostic tool for detecting live pathogens in food technology arabic 32 English 130
Marwan Draid(12-2016)

The present study aimed at evaluation of the immuomodulatory effect of sulphadimidine by investigating its ability to affect constitutive expression of MHC-II molecules on B-cells, which are one determinant of antigen presentation and the vigor of immunity. Three groups of broiler chicken were used, the first two groups received 100 mg/kg and 200 mg/kg sulfadimidine, respectively, in drinking water; while the third group was left as control. Bursal samples were taken after 7 and 14 days of treatment and checked for MHC-II gene expression by RT-PCR technique. The obtained results demonstrate that oral administration of sulphadimidine inhibited the expression of MHC-II mRNA in bursal of Fabricius of broiler chicken in a dose and age dependent manner. This finding is of clinical importance as many farmers routinely add sulpha drugs to drinking water as a prophylactic measure against some infections; yet the data presented in this study doesn't recommend this managing behavior. arabic 11 English 83
Marwan Draid(5-2012)

ABSTRACT Background: The objective of this study was to evaluate changes of haematological and biochemical parameters in Hejazi goats under synchronization protocols treatment. Methods:Forty fertile and healthy female Hejazi goats were divided equally and randomly into four groups (n=10). All synchronization protocols started 7 days after the first 125 µg Cloprostenol injection (i.m.). The first group received CIDR treatment for 7 days only and followed by injection of second dose of PGF2α and 400 IU of PMSG, and the second group received CIDR treatment for 14 days and followed by injection of second dose of PGF2α and 400 IU of PMSG, and the third group received injection of 0.004 µg of synthetic GnRH analogue for 7 days and followed on day 14 by a second injection of 125 µg PGF2α followed by second injection of 0.004 µg of synthetic GnRH analogue, the fourth group received two doses of 125 µg PGF2α with14 days interval. Blood samples were collected from all of the does for biochemical analysis. Results: Observations showed no external or behaviour changes recorded and slight changes in all groups in diagnostic parameters. The conception rate tended to be decrease specific with increase in other days Conclusions: This study showed that monitoring the biochemical diagnostic parameters in the four groups going different protocols we didn’t record any significant different (P>0.05). The current study suggests that CIDR treatment for short and long are safe in Hejazi doe
Marwan M. Draid, Abdurraouf O. Gaja, Hoda R. Shnaishah(6-2021)