Several molecular techniques that are commercially described in the literature for early detection of blood stream infection in attempting to overcome the limitations of the gold standard blood culture. SepsiTest™ is aCE marked commercial platform that has been described in the literature. However, there were no studies addressing the accuracy of the test as a diagnostic platform for detection bacteraemia in whole blood samples. The study, conducted to investigate and discuss the strategy of SepsiTest™ in comparisonto our previously validated real-time PCR based assays (BactScreen™) and the previously published “in-house” minor groove binder (MGB)-based all bacteria assay. The three assays showed different sensitivity patterns for detecting bacteria in blood stream pathogens in favouring of using BactScreenTMtest. SepsiTest™ could be valuable but their lowest sensitivity in addition to their use of the unspecific SYBR Green fluoresce dye that question its diagnostic accuracy could be used as a last choice molecular diagnostic technique for detection of bacteraemia in whole blood samples. However, SepsiTest™ strategy rather, may provide useful diagnostic tool for detecting live pathogens in food technology arabic 32 English 130
Marwan Draid(12-2016)

The present study investigated the effects of an aqueous extract of green tea (Camellia sinensis) on kidney functions through Urea, uric acid and creatinine measurement within male Wistar albino rats intoxicated with nicotine. Forty male Wistar albino rats were randomized into four groups of ten. Over a 28 day period the control group received daily subcutaneous dose of saline (10 ml) and drank only water, the green tea group received subcutaneous dose of saline (10 ml) and drank only green tea, the nicotine group received subcutaneous dose of nicotine (10 ml saline with 3mg/kg weight of nicotine) and drank only water, and the green tea with nicotine received subcutaneous dose of nicotine (10ml saline with 3mg/kg weight of nicotine) and drank only green tea. Urea, uric acid and creatinine were measured by ultraviolet spectrophotometer utilizing standard methods. No differences were observed between the control and green tea only groups, and no differences were found for creatinine levels between any of the groups. The Nicotine group had urea and uric acid concentrations significantly (P < 0.05) greater than those of the control, green tea only groups, and the green tea with nicotine groups. The results of the present study indicate that an aqueous extract of green tea reduced the effects of nicotine on urea and uric acid concentrations. arabic 10 English 59
Marwan Mustafa Ali Draid(6-2016)

Electrical field stimulation (EFS) induces frequency-dependent contractions of the longitudinal muscle of isolated quail rectum which were sensitive to tetrodotoxin. The aim of the present study was to investigate whether purinergic neurons are implicated in the response to nerve stimulation. arabic 18 English 103
M. Draid(4-2007)