Paratuberculosis (Johne's disease) was suspected in a herd of approximately 033 sheep after weight loss and scouring had increased in adult animals despite repeated treatment with anthelmintics, antibiotics, multivitamins and minerals. The herd is located near Tarhouna city. Herd history revealed that a total of 60 ewes showed clinical symptoms and deaths during the last two years. The last case that we attended was submitted to the National Center of Animal Health (NCAH) for a detailed laboratory examination. Gross pathological and histological examination of tissue samples revealed results that were highly comparable with Johne's disease. A definitive diagnosis was made only by histopathological identification of Mycobacterium paratuberculosis in the intestines using Ziehl-Neelsen stain. This is the first documented case of M. paratuberculosis in sheep in Libya.
Abdulwahab Kammon(1-2022)

A study was conducted to determine the effect of three native plants from El-Jabal al ELAkhdar, (Libya) on hematological profile, cholesterol and immune response to ND vaccine in broiler chickens. A total of 1260 one-day-old male Cobb chickens were used in the experiment. Chickens were assigned to 7 treatment groups (6 replicates per treatment). The dietary treatments included basal diet with no additives (control), and 6 other dietary treatments (Arbutus pavarii, Salvia officinalis and Zizyphus Vulgaris) each of which was added at the rate of 0.5g and 1g/kg of basal diet. Results explicitly revealed that no significant change in TLC between the treated and control groups has been recorded. A significant heteropenia was recorded at the third week of treatment with 1% A. Pavarii 0.5 S. Officinalis and 0.5 Z. Vulgaris when compared to control group. However, the lymphocytic count show a significant increase (P≤0.05) in all treated groups compared to the control groups. Immunologically, an enhanced humeral immune response was very obvious based upon the significant elevation of antibody titer at the third week of treatment for all groups received 1 % of the three types of plants. Interestingly , cholesterol levels were significantly elevated at the six week of treatment with 0.5 % Z. Vulgaris while highest decline in cholesterol levels were recorded in group that have received 0.5 S. Officinalis.
ِAbdulatif A. Asheg(3-2015)

Abstract The use of antibiotics as growth promotors to enhance animal production is banned in many countries around the world due to antimicrobial resistance. There is a need therefore, for new alternatives to antibiotics in medicine and veterinary medicine practices. The antibacterial activity of clove oil and some antibiotics were tested in vitro against three isolates of E. coli {1 avian pathogenic (E. coli 6.2) and 2 non-pathogenic (E. coli 6.1 and E. coli X)}, Salmonella enteritidis and Salmonella spp., by disk diffusion and minimum inhibitory concentration (MIC) methods. All bacteria tested showed intermediate susceptibility to clove oil using disk diffusion method except the non-pathogenic E.coli 6.1. The inhibition zones measured were 0mm, 12mm, 13mm, 15mm and 15mm for E. coli 6.1, E. coli 6.2, E. coli X, Salmonella enteritidis and Salmonella spp., respectively. E. coli 6.1 was resistant to Ampicillin, and Lincomycin with multi antibiotic resistance (MAR) index of 0.2. E. coli 6.2 was resistant to Ampicillin, Doxycyclin and Lincomycin with MAR index of 0.3. E. coli X was resistant to Ampicillin and Colistin with MAR index of 0.2. Salmonella enteritidis was resistant to Amoxycillin/Clavulinic acid, Ampicillin and Lincomycin with MAR index of 0.3. Salmonella spp was susceptible only to Neomycin but was resistant to 9 out of 10 antibiotics with very high MAR index of 0.9. The MICs of clove oil were 6.25mg/ml for the avian pathogenic E. coli and 3.12mg/ml for non-pathogenic E. coli and Salmonella spp. and 12.5mg/ml for Salmonella enteritidis. It is concluded that clove oil has promising antibacterial activity and more studies are needed to investigate its in vivo activity as alternative to antibiotics in poultry arabic 13 English 85
Abdulwahab Kammon, Ali Almaeyoufi , Abdulatif Asheg(7-2019)