Peste des Petits Ruminants (PPR) is considered one of the most important transboundary animal diseases (TADs) with socio-economic impacts on national and international levels. During 2015-2016 a total of 690 serum samples were collected from unvaccinated domestic ruminants of which 555 sheep, and 135 goats representing teen provinces distributed in four Libyan branches (Green Mountain, Benghazi, West Mountain and Sabha). The sample were analysed at IZSAM, Teramo, Italy, by using competitive ELISA, IDvet innovative diagnostics (IDvet, 310 rue Louis Pasteur-34790 Grabels, France). The overall sero-prevalence rate (SPR) of PPR antibodies was estimated to be 41% (95% CL: 36% to 46%) among sheep and 39% (95% CL: 28% to 45%) among goat. A chi-square test was used to evaluate the probability of differences observed among SPR of infection. The results showed that the SPR of PPR was significantly (P= 0.015) higher in adult animals 41% (95% CL= 37%-46%) than in the young 24% (95% CL= 17%- 32%). The highest SPR 75% (95% CL= 61%-85%) was recorded in Sabha province (Southern Libya) which highlighted statistically difference (P= 0.00001). The preliminary results of the present study could be useful to better focus on specific area of Libya to improve understand and evaluate the risk factors for disease spreading and to plan disease control activities as requested by FAO/OIE (PPR Global Control and Eradication Strategy). arabic 19 English 100
Abdusalam Sharef Abdusalam Mahmoud(1-2020)

Abstract Background: Epizootic haemorrhagic disease (EHD) is a vector-borne viral disease of domestic and wild ruminants. Epizootic haemorrhagic disease virus (EHDV) is transmitted by Culicoides spp. EHDV is a member of the Orbivirus genus within the Reoviridae family. It shares many morphological and structural characteristics with other members of the genus, such as the bluetongue virus, African horse sickness virus, and equine encephalosis virus. Aims: The purpose of our study was to investigate the epidemiological situation of EHDV in Libya in order to gain some knowledge about the presence of this virus in the country. Methods: In this study, we investigated the seroprevalence of EHDV in Libya, testing 855 blood samples collected during 2015. The samples were collected from domestic ruminants (cattle, sheep, and goats) originating from 11 provinces of Libya. Sera were tested by competitive enzyme-linked immunosorbent assays and positive samples confirmed by serum neutralization test. Results: The overall seroprevalence of EHDV was estimated to be 4% (95% confidence intervals = 2.8%–5.4%). Small ruminant seroprevalence was significantly (p = 0.016) higher than that found in cattle. Neutralizing antibodies against EHDV-6 were detected in a sheep from the western region of Libya. Conclusion: This study suggests that EHDV has circulated or is circulating in Libya, and sheep could play an important role in the epidemiology of EHDV, and the virus may still be circulating in North Africa. Keywords: EHD, EHDV-6, Seroprevalence, Libya.
Abdusalam Sharef Abdusalam Mahmoud(6-2021)

A serological survey was carried out in Libya to investigate the circulation of Rift Valley fever virus (RVFV) among domestic ruminants. A total of 857 serum samples were collected from year 2015 to 2016 in eleven provinces of Libya belonging to five branches of the country. Samples were tested for RVFV antibodies using a competitive EnzymeLinked Immunosorbent Assay (c-ELISA). Antibodies specific for RVFV were not detected in any of the 857 samples. However, a statistical analysis was carried out to assess the maximum expected number of infected animals and the maximum expected prevalence of RVFV among Libyan ruminants’ populations according to the sampled population. The overall maximum expected prevalence was estimated to be 1.8% for cattle and 0.4% for small ruminants. Results seem to exclude the circulation of RVFV, however, a surveillance plan should be implemented in areas at risk of RVFV introduction. arabic 10 English 55
Abdusalam Sharef Abdusalam Mahmoud, (6-2018)