Abstract This study is to identify some mutations in exons 23, 24, 26 A+B, and 26 A of the factor VIII gene that causes hemophilia A among Libyan patients. Forty-three patients (40 males, 3 females), 10 carrier, and 10 control (8 males, 2 females) were enrolled in this study. The total number were 63 samples for each exon. Blood samples were collected from Libyan patients (Department of Paediatric Hematology, Tripoli Medical Center), and kept in freezer at -20 Cº. The blood samples were transported to DNA laboratory in the Genetic Engineering Department and Human Tissues Department Biotechnology Research Center, Twesha- Libya. Genomic DNA was extracted from whole blood and DNA was visualized by Agarose Gel Electrophoresis, and DNA concentration was measured by Spectrophotometer. DNA was amplified by polymerase chain reaction (PCR) technique. Mutations were identified by restriction enzymes Taq I and Xba I. Taq I was used to detect nonsense mutations in Exon 23, 2147 [ Arg Stop codon (Term)], Exon 24, 2209 [ Arg Stop codon (Term)], and Exon 26 A+B, 2307 [ Arg Stop codon (Term)]. Our result showed no mutations were detected by using restriction enzyme Taq I in Exons 23, codon 2147, Exon 24, codon 2209, and Exon 26A+B, codon 2307. But by using restriction enzyme Xba I, we found six insertion mutations in Exon 26A, 3919 (ins GA) 11.3 % of 53 patients, and two partial deletion mutations in Exon 23 (3.8 % of 53 patients) refer to area failed to be amplified by PCR.
نادية نور الدين الصربوط (2010)

تؤثر المحاصيل البقولية على خصوبة الأراضي الزراعي حيث ترفع من المحتوى النيتروجيني للتربة بواسطة بكتيريا العقد الجذرية التي تعيش في جذور المحاصيل البقولية بتعايش تبادل منفعة، ويعتبر البرسيم الحجازي من أقدم محاصيل العلف البقولية المستدامة في العالم وهو ذو قيمة غذائية عالية. إن التفاعلات المتداخلة بين المجموعة الحيوانية المفصلية والمخلوقات الدقيقة والتربة تعمل على خصوبة وتركيب التربة في وجود الظروف البيئية المناسبة من درجة الحرارة والرطوبة والأس الهيدروجيني. هدفت هذه الدراسة إلى التعرف على المجموعات الرئيسية للمفصليات المتلازمة، وتأثير بعض العوامل المتداخلة على توزيعها وكثافتها، والعلاقات المتداخلة بين وفرة الحلم و ذوات الذنب القافز والمفصليات الأخرى بالنظام البيئي الزراعي للبرسيم الحجازي في كلا من موقع تاجوراء وعين زاره ووادي الربيع. بينت النتائج مفصليات الأرجل في التربة في مواقع الدراسة كانت شائعة في الطبقة العليا حتى عمق 10 سم في حقول البرسيم، وتميز موقع عين زاره بأعلى كثافة من مفصليات التربة المتلازمة مع البرسيم الحجازي يليه موقع تاجوراء ثم موقع وادي الربيع. عملية إفتراس الحلم على ذوات الذنب القافز كانت بمعنوية عالية في النظام البيئي الزراعي للبرسيم الحجازي. كما بينت النتائج بأن عوامل الموقع ودرجة الحرارة والرطوبة النسبية هي المتغيرات الأساسية في التسبب في ارتفاع الكثافة العددية لمفصليات التربة في النظام البيئي الزراعي للبرسيم الحجازي. Abstract Leguminous crops have an fertile effects on agricultural lands, mainly because of soil nitrogen contents produced by bacterial nodes as commensalism. Alfalfa is considered one of the oldest sustainable legume crop of nutritious value in the world. Life interactions among arthropods groups, microorganisms and soil working together for soil fertility and structure, in presence of suitable environmental factors mainly temperature, relative humidity and Ph. The main goals of this study are to identify soil arthropods, effect of interaction factors on the distribution and abundance of soil arthropods, interaction factors among soil collembolan and mites, and other soil arthropods associates with alfalfa agrecosystem in three locations of Tripoli area: Tajoura Ain Zara and wad Alrabee. Results revealed that alfalfa soil arthropods are common in the upper soil layer to a depth of 10 cm. Ain Zara showed the highest soli arthropod density than Tajoura and Wadi Alrabee. Predation of mites on collembolans was highly significant in Alafia agroecosystem. Data also indicated that location characteristics factors, temperature, year seasons, relative humidity were the main significant reasons for the relative abundance of soil arthropods in the alfalfa agroecosystem.
صبرية علي فرج رافع (2016)

Efficient extraction of genomic DNA (gDNA) from biological materials found in harsh environments is the first step for successful forensic DNA profiling. This study aimed to evaluate two methods for DNA recovery from animal tissues (livers, muscles), focusing on the best storage temperature for DNA yield in term of quality, quantity, and integrity for use in several downstream molecular techniques. Six male Swiss albino mice were sacrificed, liver and muscle tissues (n=32) were then harvested and stored for one week in different temperatures, -20C, 4C, 25C and 40C. The conditioned animal tissues were used for DNA extraction by Chelex-100 method or NucleoSpin Blood and Tissue kit. The extracted gDNA was visualized on 1.5% agarose gel electrophoresis to determine the quality of gDNA and analysed spectrophotometrically to determine the DNA concentration and the purity. Both methods, Chelex-100 and NucleoSpin Blood and Tissue kit found to be appropriate for yielding high quantity of gDNA, with the Chelex100 method yielding a greater quantity (P < 0.045) than the kit. At -20C, 4C, and 25C temperatures, the concentration of DNA yield was numerically lower than at 40C. The NucleoSpin Blood and Tissue kit produced a higher (P=0.031) purity product than the Chelex-100 method, particularly for muscle tissues. The Chelex-100 method is cheap, fast, effective, and is a crucial tool for yielding DNA from animal tissues (livers, muscles) exposed to harsh environment with little limitations.
Huda H. Al-Griw, Zena A. Zraba, Salsabiel K. Al-Muntaser, Marwan M. Draid, Aisha M. Zaidi, Refaat M. Tabagh , Mohamed A. Al-Griw(8-2017)