أُجريت هذه الدراسة على السلحفاة البحرية ضخمة الرأس Caretta caretta خلال الفترة من 2006 الى 2010 باستثناء سنة 2008. وذلك بمنطقتي القبيبة والثلاثين غرب مدينة سرت بالمنطقة الوسطى لليبيا. الهدف العام لهذه الدراسة كان حول سلوك التعشيش للسلحفاة البحرية ضخمة الرأس وتاثير بعض العوامل البيئية والبيولوجية على نجاحه. خلال هذه الدراسة تم حصر سبعة وعشرون نوعاً من النباتات الحولية والشجيرية وخصوصاً الملحية. أمتدت مواسم التعشيش من نهاية شهر مايو حتى شهر سبتمبر من كل سنة وتمت عملية المسح يومياً في الصباح الباكر سيراً على الاقدام، و قد استخدم لذلك نموذج خاص إشتمل على التاريخ والموقع ونوع الاثر وعمق العش وقياسات السلحفاة من خلال الصدفة، كما تم نقل بعض الاعشاش الى مكان محمي باستخدام حافظات خاصة. اوضحت نتائج الدراسة وجود تباين في كثافة التعشيش بين منطقتي الدراسة حيث كانت أعلى كثافة تعشيش خلال موسم 2007 بينما أقل كثافة كانت سنة 2010، وكان الفرق معنوياً في منطقة القبيبة حيث أن كثافة التعشيش تأثرت سلباً بإنشاء محطة توليد الكهرباء بالمنطقة. كما خلصت نتائج هذه الدراسة الى عدم وجود علاقة بين طول الصدفة وعدد البيض، وكذلك عدم وجود فروق معنوية بين عدد البيض في كل حضنة وتاريخ التعشيش في الموسم الواحد. كما أكدت هذه الدراسة وجود فارق معنوي في نسبة الفقس بين الاعشاش الطبيعية والمنقولة بحيث كانت نسبة الفقس في الأعشاش المنقولة أعلى منه في الطبيعية كما بينت هذه الدراسة بأن نسبة الفقس لا تتأثر بالمسافة بين العش وخط المد.
المختار موسى عامر سعيد (2015)

Environmental toxicants such as chemicals, heavy metals, and pesticides have been shown to promote transgenerational inheritance of abnormal phenotypes and/or diseases to multiple subsequent generations following parental and/ or ancestral exposures. This study was designed to examine the potential transgenerational action of the environmental toxicant trichloroethane (TCE) on transmission of liver abnormality, and to elucidate the molecular etiology of hepatocyte cell damage. A total of thirty two healthy immature female albino mice were randomly divided into three equal groups as follows: a sham group, which did not receive any treatment; a vehicle group, which received corn oil alone, and TCE treated group (3 weeks, 100 μg/kg i.p., every 4th day). The F0 and F1 generation control and TCE populations were sacrificed at the age of four months, and various abnormalities histpathologically investigated. Cell death and oxidative stress indices were also measured. The present study provides experimental evidence for the inheritance of environmentally induced liver abnormalities in mice. The results of this study show that exposure to the TCE promoted adult onset liver abnormalities in F0 female mice as well as unexposed F1 generation offspring. It is the first study to report a transgenerational liver abnormalities in the F1 generation mice through maternal line prior to gestation. This finding was based on careful evaluation of liver histopathological abnormalities, apoptosis of hepatocytes, and measurements of oxidative stress biomarkers (lipid peroxidation, protein carbonylation, and nitric oxide) in control and TCE populations. There was an increase in liver histopathological abnormalities, cell death, and oxidative lipid damage in F0 and F1 hepatic tissues of TCE treated group. In conclusion, this study showed that the biological and health impacts of environmental toxicant TCE do not end in maternal adults, but are passed on to offspring generations. Hence, linking observed liver abnormality in the offspring to environmental exposure of their parental line. This study also illustrated that oxidative stress and apoptosis appear to be a molecular component of the hepatocyte cell injury.
Mohamed A. Al-Griw , Soad A. Treesh, Rabia O. Alghazeer, Sassia O. Regeai (7-2017)

Efficient extraction of genomic DNA (gDNA) from biological materials found in harsh environments is the first step for successful forensic DNA profiling. This study aimed to evaluate two methods for DNA recovery from animal tissues (livers, muscles), focusing on the best storage temperature for DNA yield in term of quality, quantity, and integrity for use in several downstream molecular techniques. Six male Swiss albino mice were sacrificed, liver and muscle tissues (n=32) were then harvested and stored for one week in different temperatures, -20C, 4C, 25C and 40C. The conditioned animal tissues were used for DNA extraction by Chelex-100 method or NucleoSpin Blood and Tissue kit. The extracted gDNA was visualized on 1.5% agarose gel electrophoresis to determine the quality of gDNA and analysed spectrophotometrically to determine the DNA concentration and the purity. Both methods, Chelex-100 and NucleoSpin Blood and Tissue kit found to be appropriate for yielding high quantity of gDNA, with the Chelex100 method yielding a greater quantity (P < 0.045) than the kit. At -20C, 4C, and 25C temperatures, the concentration of DNA yield was numerically lower than at 40C. The NucleoSpin Blood and Tissue kit produced a higher (P=0.031) purity product than the Chelex-100 method, particularly for muscle tissues. The Chelex-100 method is cheap, fast, effective, and is a crucial tool for yielding DNA from animal tissues (livers, muscles) exposed to harsh environment with little limitations.
Huda H. Al-Griw, Zena A. Zraba, Salsabiel K. Al-Muntaser, Marwan M. Draid, Aisha M. Zaidi, Refaat M. Tabagh , Mohamed A. Al-Griw(8-2017)