one of the main factors that affect the success of the cast crown is the accuracy of the internal and marginal fit, a well-fitting crown reduces the chance for recurrent caries and periodontal disease, whereas the space between a poorly-fitting crown and tooth preparation enables accumulation of bacterial plaque, the use of paint- on die spacing technique to produce the cement gap and subsequently to improve the seating of the cast crowns has become quite popular in the recent years. The purpose of the study is to compare the marginal gap and the internal fit produced by two paint-on die spacing materials (Manicure and paint on die spacer ). Twenty four identical stone dies were made, 12 were coated with paint- on die spacer material, and 12 were coated with nail varnish material, the dies coated with die spacer were divided into three groups (DA,DB,DC), and the dies coated with nail varnish were also divided into three groups (VA,VB,VC), groups DA and VA were coated with one layer, DB and VB were coated with two layers, DC and VC were coated with three layers, for both groups wax patterns were made and casted using the lost waxing technique and all steps were standardized, impression replicas of the casted crowns were made and sectioned by means of the impression replica technique, measurements were taken using Scanning Electron Microscope, from 4 points (marginal gap, mid-axial, axio-occlusal and mid-occlusal). There was no statistical significant difference in accuracy between crowns fabricated on die spacer coated dies and crowns fabricated on nail varnish coated dies. with the crowns fabricated on die spacer coated dies being slightly more accurate.
ABDUSSALAM ELJABALI(12-2019)

The most common preparation of tocotrienols is the tocotrienol-rich fraction (TRF). This study aimed to investigate whether TRF induced liver Nrf2 nuclear translocation and influenced the expression of Nrf2-regulated genes. Methods: In the Nrf2 induction study, mice were divided into control, 2000 mg/kg TRF and diethyl maleate treated groups. After acute treatment, mice were sacrificed at specific time points. Liver nuclear extracts were prepared and Nrf2 nuclear translocation was detected through Western blotting. To determine the effect of increasing doses of TRF on the extent of liver nuclear Nrf2 translocation and its implication on the expression levels of several Nrf2-regulated genes, mice were divided into 5 groups (control, 200, 500 and 1000 mg/kg TRF, and butylated hydroxyanisole-treated groups). After 14 days, mice were sacrificed and liver RNA extracted for qPCR assay. Results: 2000 mg/kg TRF administration initiated Nrf2 nuclear translocation within 30 min, reached maximum level around 1 h and dropped to half-maximal levels by 24 h. Incremental doses of TRF resulted in dose-dependent increases in liver Nrf2 nuclear levels, along with concomitant dose-dependent increases in the expressions of Nrf2-regulated genes. Conclusion: TRF activated the liver Nrf2 pathway resulting in increased expression of Nrf2-regulated cytoprotective genes.
Ahmed Elbadri M Atia(9-2020)

Background: Libya has been malaria-free since 1973. The risk of malaria re-introduction to Libya is increasing because of the increase in imported malaria cases due to immigration to Libya from countries where malaria is endemic. Cases are mainly due to P. falciparum and Sub-Saharan Africa is the most common origin. This study was conducted to investigate the prevalence of malaria positive cases among illegal immigrants in the southern region of Libya. Methods: A prospective, observational, multi-center study was conducted. Three hundred and three illegal immigrants from 12 different countries were included. Enzyme-linked immunosorbent assay was used to detect the presence of serum malaria antibodies. Results: A total of 303 immigrants were included in the study with mean age of 25.78±5.92 years. Of them, 195 tested positive for malaria antibodies. Two hundred and sixty nine of the participants came from Brak Al-Shati, 16 from Sebha and 18 from Bergan centers, with 175, 15 and 5 positive cases, respectively. Most of the positive cases (172) arrived in Libya within 1-10 months. The highest number of positive cases (70) came from Ghana followed by (40) from Niger and (39) from Bangladesh. Conclusions: Illegal immigration is a major malaria re-introduction route. The issue of illegal immigration have to be treated urgently to stop the huge influx of illegal immigrants and increase the surveillance activities of infectious diseases in order to keep Libyan territories as a malaria-free lands. Key words: Illegal immigration, Malaria, Libya.
Fadwa Jamaledden Mustafa Kamel Mahanay, Badereddin Bashir K. Annajar, Asma A Ali Oun(11-2021)