The most common preparation of tocotrienols is the tocotrienol-rich fraction (TRF). This study aimed to investigate whether TRF induced liver Nrf2 nuclear translocation and influenced the expression of Nrf2-regulated genes. Methods: In the Nrf2 induction study, mice were divided into control, 2000 mg/kg TRF and diethyl maleate treated groups. After acute treatment, mice were sacrificed at specific time points. Liver nuclear extracts were prepared and Nrf2 nuclear translocation was detected through Western blotting. To determine the effect of increasing doses of TRF on the extent of liver nuclear Nrf2 translocation and its implication on the expression levels of several Nrf2-regulated genes, mice were divided into 5 groups (control, 200, 500 and 1000 mg/kg TRF, and butylated hydroxyanisole-treated groups). After 14 days, mice were sacrificed and liver RNA extracted for qPCR assay. Results: 2000 mg/kg TRF administration initiated Nrf2 nuclear translocation within 30 min, reached maximum level around 1 h and dropped to half-maximal levels by 24 h. Incremental doses of TRF resulted in dose-dependent increases in liver Nrf2 nuclear levels, along with concomitant dose-dependent increases in the expressions of Nrf2-regulated genes. Conclusion: TRF activated the liver Nrf2 pathway resulting in increased expression of Nrf2-regulated cytoprotective genes.
Ahmed Elbadri M Atia(9-2020)

Background: Management of partially edentulous patients with hard and soft tissue undercuts is complicated, and the use of flexible dentures for these cases offers dual advantages of aesthetics and flexibility. However, research shows a varying preference for flexible dentures among general dental practitioners, prosthodontists, and other dental specialties. Objectives: The aim of this study was to survey the attitude and awareness of a sample of Libyan dental clinicians towards advantages, disadvantages, indications, and contraindications of flexible dentures. Material and methods: A descriptive cross-sectional study was conducted among 250 dentists currently practicing in different cities of Libya. Only 218 dentists responded. Using Google form software, a self-administrable e-questionnaire consisting of thirty-one multiple choice questions with "yes," "no" or "not sure" answers encompassing major aspects of flexible dentures was conducted through an online survey. Results: Out of 250 Libyan dentists to whom the questionnaire was sent, only 218 dentists responded and our results revealed that the respondents were females more than males with a ratio (2:1) and more than half of them were of age group (35-60 years) work in governmental dental practice with more than ten years of experience and 34.9% of them prefer and often offer flexible dentures to their patients. The majority of respondents with a long-term success of the flexible denture were prosthodontists who had more than ten years of experience with a correct response rate of more than 50% of questions that reflected their attitude and knowledge. Conclusions: Despite the fact that flexible dentures are not taught in Libyan universities of dentistry, about more than one-third of respondents prefer and recommend this treatment to their patients, and the long-term success of these prostheses was dependent on clinicians' education and their clinical experience.
Yusra Muftah Saad Elfaidy, Warida Abdalla Elnaihoum, Aseidah Abdullah Elgotrany(4-2022)

one of the main factors that affect the success of the cast crown is the accuracy of the internal and marginal fit, a well-fitting crown reduces the chance for recurrent caries and periodontal disease, whereas the space between a poorly-fitting crown and tooth preparation enables accumulation of bacterial plaque, the use of paint- on die spacing technique to produce the cement gap and subsequently to improve the seating of the cast crowns has become quite popular in the recent years. The purpose of the study is to compare the marginal gap and the internal fit produced by two paint-on die spacing materials (Manicure and paint on die spacer ). Twenty four identical stone dies were made, 12 were coated with paint- on die spacer material, and 12 were coated with nail varnish material, the dies coated with die spacer were divided into three groups (DA,DB,DC), and the dies coated with nail varnish were also divided into three groups (VA,VB,VC), groups DA and VA were coated with one layer, DB and VB were coated with two layers, DC and VC were coated with three layers, for both groups wax patterns were made and casted using the lost waxing technique and all steps were standardized, impression replicas of the casted crowns were made and sectioned by means of the impression replica technique, measurements were taken using Scanning Electron Microscope, from 4 points (marginal gap, mid-axial, axio-occlusal and mid-occlusal). There was no statistical significant difference in accuracy between crowns fabricated on die spacer coated dies and crowns fabricated on nail varnish coated dies. with the crowns fabricated on die spacer coated dies being slightly more accurate.
ABDUSSALAM ELJABALI(12-2019)