Measles, Mumps ,and rubella (MMR) are serious diseases that can lead potentially to disability and death. The study aimed to determine seroprevalence rates of antibody levels against MMR among Libyan children at school entry. This was a cross sectional study undertaken in Tripoli, from August to September 2016. Children of school-entry age (> 5 to ≤ 7 years) were randomly recruited at vaccination centers. Sera were tested for antibodies againstMMR. Data analyzed by SPSS program version 20. Out of 546 children, 290 were males. IgG antibodies against measles, mumps, and rubella were positive in 513 (94%), 436 (80%), 538 (98.5%) children, respectively. Seropositivity rates in Libyan children at school entry is protective and this is similar in so many other studies. This indicates that MMR vaccine program is effective in strengthening herd immunity in the Libyan community. arabic 19 English 111
Mohamed Abugalia, A Bashein(1-2021)

Every organism has deoxyribonucleic acid (DNA) within their cells. DNA is a complex molecule that contains all of the information needed to build and maintain living organisms. Extraction of deoxyribonucleic acid (DNA) is one of the most basic and critical steps affecting molecular-based techniques in the study of DNA that allow vast advances in genetic, molecular biology, biotechnology, forensic, and bioinformatics laboratories. Therefore, researchers have been used different modified and optimized protocols for efficient genomic DNA extraction from biological samples. Due to the high amount of genetic material in whole blood samples so it's one of the main sources used to obtain DNA and there are many different protocols available in this issue. In the present study, we optimized, evaluated by comparison to phenol-chloroform (traditional method) and silica column (QIAamp DNA Blood Mini Kit) DNA extraction procedures. The extracted DNA by these protocols was analyzed according to their time consuming, quality, quantity, cost and toxicity. Extracted DNA with current protocol was qualified using gel electrophoresis, Nanodrop spectrophotometric analysis. Our results showed that there are not significantly differences between these methods about DNA Purity (A260/A280) and DNA yield (ng DNA/μl). In addition, phenol/chloroform (traditional method) was the most toxic method; it takes more time but cheaper than other method; it yielded reasonably good quantities of good quality DNA and would be suitable for large-scale genotyping of blood samples. The silica column method (QIAamp DNA Blood Mini Kit) was the most expensive among the other method but the least extraction time was required and it was the safest method. arabic 11 English 69
Ghada Salem, Ahmed Zaid(1-2018)

Asthma has been considered the most common chronic respiratory disease affecting children and is involved in .different diagnoses of acute bronchiolitis in first year of life Therefore, this research has been conducted to explore the relationship between asthma and bronchiolitis on known asthmatics aging from 6months old to 16 years old frequenting the asthma clinic Tripoli Children Hospital for .)regular follow ups for 3 months (January, February, and march of 2004 The aim of this study: To ascertain the percent (prevalence)of bronchiolitis patients who will develop]e asthma .symptoms later on Conclusively there has been a strong relationship between asthma and bronchiolitis in a way that bronchiolitis pre- .disposes to asthma in 53.5% of patients especially in whom had a family history of allergy
Hisham Mukhtar Alrabty(4-2020)