Aim: The aim of the study was to assess the success and possible complications of closed reduction (CR) treatment of developmental hip dysplasia (DHD) in late‑diagnosed children and explores its relation to the acetabular index (AI) measurement prior to treatment. Patients and Methods: Twenty‑three consecutive patients with dislocated hips, 16 unilateral and 7 bilateral (30 hips), were retrospectively included in the study. They were admitted to the specialist pediatric orthopedic unit of the University Hospital (Tripoli Medical Center) in Tripoli, Libya. There were 21 females and 2 males with an average age at diagnosis of 17 months (range from 14 to 31 months). Their average follow‑up period was 3 years (2–5 years), and none of them received treatment prior to diagnosis. All patients received prior inpatient skin traction for at least 2 weeks followed by CR with soft tissue release (adductor tenotomy), hip spica applied and maintained for an average of 3 months. Patients who had a failure of reduction or resubluxation at follow‑up went for open reduction and a reconstruction procedure. Results: CR was successful in 27 hips (90%), failed in 3 (10%) other, the average age of the successful reduction group was 20.5 months, while that of the open reduction group, it was 23 months (Р = 0.25). The average AI of the CR group was 39.0°, while that of the open reduction group, it was 42.7° (Р = 0.15); 6.7% of patients with an AI of 40° had a failure of CR of the hip (Р = 0.46). No complications of treatment were recorded at follow‑up. Conclusion: Staged CR of DHD in older children in the hands of experienced specialists is still a valid means of their treatment, especially in developing countries with limited resources. There is a relatively higher failure rate of CR, the older the child is and the higher the AI. arabic 11 English 78
Nabil Alageli, Majdi Alakkari(4-2021)

Preterm neonates are highly vulnerable and most susceptible to Infections. Gram-negative bacterial (GNB) infection is an increasing problem among hospitalized neonates. It is showing periodic and geographic variations in the distribution and antibiotics resistance which necessitate continuous surveillance. In present study surveillance of Gram-negative bacterial colonization and infection among preterm neonates was carried out between July, 2008 and January, 2009 at AL Jala Hospital of Obstetric and Gynecology, Tripoli. This study aimed to determine the prevalence of Gram negative infections among preterm neonates, correlate colonization with the onset of subsequent infection, and to determine the antibiotics susceptibility of Gram-negative isolates. Surveillance swabs from mouth, nose, rectum, axilla and umbilicus were collected from 112 preterm neonates twice at first week, the first swab was taken before the preterm receive any antibiotics, then once per week. Clinical samples from preterm neonates who developed infection were collected according to the site of suspected infection. Samples transport, isolation and identification of GNB were conducted according to standard microbiological methods. Infection is the cause of death in 25% of cases. 19.6% of neonates colonized at the first day, 63.2% at third day and 66.7% at the second week. 25 of 74 colonized neonates and one of 38 noncolonized neonates developed infection. Rectum was the commonest site of colonization. A. baumannii is a permanent colonizer, K. pneumoniae and E. coli are early colonizers and E. cloacae and Pseudomonas spp. are late colonizers. Isolates specially K. pneumoniae and E. coli showed high resistance to most used antibiotics mainly ampicillin and gentamicin. 26 (23%) neonates developed infection mainly caused by E. coli and K. pneumoniae. Only eight cases using microbiological culture proved infections. In conclusion Gram-negative infection could be a major cause of death among preterm neonates. Acquisition of GNB increased with hospitalization and it is an important step in developing infection. Preterm neonates were heavily colonized at 3rd day of hospitalization by most GNB. K. pneumoniae and E. coli were found to be the most resistant strain to antibiotics and were associated with high rate of infection. Rectum could be used as a surveillance site instead of the other sites.
زينب عبد الله كريمه (2013)

Factor V Leiden G1691A (FVL) and Factor II prothrombin G20210A (PGM) mutations are the leading causes of thrombophilia. In this study, we have investigated the prevalence of the FVL G1691A and PGM G20210A single nucleotide polymorphisms (SNPs) among Libyan deep vein thrombosis (DVT) and myocardial infarction (MI) patients. SNP genotyping was performed using high-resolution melt analysis (HRM) and DNA sequencing. Biochemical parameters conducted on 112 males and 93 females showed no significant difference in means between the control group and the deep vein thrombosis and myocardial infarction groups. For Factor V Leiden, 40 samples were genotyped. Of the 40 samples, 6 (15.0%) of them were heterozygous and no one was homozygous. As for Factor II SNP, 59 samples were genotyped and only 2 (3.3%) were heterozygous. All the heterozygous samples showed 100% concordance between the HRM-PCR and DNA sequence analysis. Our study showed, for the first time, that both the FVL and PGM mutations are present among Libyan DVT and MI patients and that the FVL mutation is significantly associated with DVT but not with MI. However, our results do not support the association of PGM G20210A mutation with DVT or MI. arabic 18 English 117
Abdulghani Msalati, Abdulla Bashein, Murad Ghrew, Khaled Sedaa, Abushawashi Ali, Ahmed Zaid, (1-2021)